anti rab 5 rabbit mab  (Cell Signaling Technology Inc)

Journal: mBio

Article Title: Herpes simplex virus diverts CIN85 endosomal cargo for exocytosis to evade antiviral responses: a novel role for the viral immediate-early protein ICP0

doi: 10.1128/mbio.02143-25

Figure Lengend Snippet: ICP0 localizes on the surface of the CIN85 structures, along with early and late endosome, autophagy, and innate immunity factors. ( A ) Vero cells were transfected with a CIN85-Flag expressing plasmid for 24 h followed by HSV-1(F) infection (10 PFU/cell). Cells were fixed at 14 h post-infection and stained with an ICP0 and a Flag antibody. Images were captured using a CSU-W1 SoRa confocal microscope. ( B and C ) Vero cells were transfected with plasmids expressing CIN85-Flag, mCherry-Rab5, mRFP-Rab7, mCherry-LC3, mCherry-p62, mCherry-ATG5, YFP-Sp100A, and CD63-EGFP or co-transfected with a CIN85-Flag-expressing plasmid and plasmids expressing the above-mentioned proteins. At 24 h post-transfection, the cells were infected with HSV-1(F) (10 PFU/cell). Cells were fixed at 14 h post-infection and probed with an ICP0 or a Flag antibody. Images were captured using a TCS SP8 STED microscope.

Article Snippet: Rab5 , Mouse , 1:1,000 , Santa Cruz , sc-46692.

Techniques: Transfection, Expressing, Plasmid Preparation, Infection, Staining, Microscopy

Journal: mBio

Article Title: Herpes simplex virus diverts CIN85 endosomal cargo for exocytosis to evade antiviral responses: a novel role for the viral immediate-early protein ICP0

doi: 10.1128/mbio.02143-25

Figure Lengend Snippet: HSV-1(F) infection induces exocytosis of CIN85 and of components colocalizing with CIN85. ( A and Β ) hTERT-HEL cells were uninfected, infected with the WT virus or various ICP0 mutants (0.5 PFU/cell). The cells were harvested at 48 h post-infection, and total EVs were analyzed for the indicated proteins. Equal amounts of proteins from total cell lysates were included as controls. ( C and D ) U2OS cells were infected with HSV-1(F), ΔICP0, and ICP0 delta244–277 virus (0.5 PFU/cell) or remained uninfected. EVs were collected at 48 h post-infection, and equal amounts were analyzed for CIN85 exocytosis. Equal amounts of proteins from total cell lysates were analyzed for CIN85, VP16 which served as a control for the infection, and β-actin which served as a loading control. ( Ε and F ) hTERT-HEL cells were infected with HSV-1(F), ΔICP0, and ICP0 delta244–277 virus (0.5 PFU/cell) or remained uninfected. EVs were collected from culture supernatants at 48 h post-infection and analyzed for Rab5, Rab7, ATG5, Sp100, p62/SQSTM1, Alix, and LC3-B. Equal amounts of proteins from total cell lysates were analyzed for the same proteins. ICP0 or VP16 was used as controls for the infection and β-actin as a loading control.

Article Snippet: Rab5 , Mouse , 1:1,000 , Santa Cruz , sc-46692.

Techniques: Infection, Virus, Control

Journal: iScience

Article Title: ALS-associated TDP-43 aggregates drive innate and adaptive immune cell activation

doi: 10.1016/j.isci.2025.112648

Figure Lengend Snippet: TDP-43 aggregates compromise intracellular vesicle integrity Airyscan and confocal micrographs (630X) illustrate TDP-43a co-localization with early endosome marker Rab5a (scale bars, 10 μm and 1 μm for insets) (A), acidified vesicle marker LysoTracker (scale-bar 15-μm and 5-μm for insets) (B), and ruptured autophagosome markers LC3B and galectin-3 (scale bar, 1 μm) (C). Representative images were acquired from n = 3 independent experiments. (D) Transmission electron micrographs depict whole-cell hMDM (top left; scale bars, 10 μm), hMDM vesicles following vehicle treatment (upper right; scale bars, 1 μm), and vesicles following TDP-43a treatment for 1-h (lower left; scale bars, 0.5 μm) and 16-h (lower right; scale bars, 1 μm). Representative vesicles are shaded yellow. Representative images were acquired from n = 2 independent experiments.

Article Snippet: Rab5a , Santa Cruz , IF: 1:100 , SC46692 , RRID: AB_628191.

Techniques: Marker, Transmission Assay